Identifcation of Trichinella taxa by ITS-1 amplicon next-generation sequencing with an improved resolution for detecting underrepresented genotypes in mixed natural infections
- DOI
- Language of the publication
- English
- Date
- 2023-12-21
- Type
- Article
- Author(s)
- Lobanov, Vladislav A.
- Konecsni, Kelly A.
- Scandrett, Brad W.
- Jenkins, Emily J.
- Publisher
- BioMed Central
Abstract
Amplicon-based next-generation sequencing (NGS) has rapidly gained popularity as a powerful method for delineating taxa in complex communities, including helminths. Here, we applied this approach to identify species and genotypes of zoonotic nematodes of the Trichinella genus. A known limitation of the current multiplex PCR (mPCR) assay recommended by the International Commission on Trichinellosis is that it does not differentiate Trichinella nativa from T. chanchalensis.
Subject
- Animal diseases
Keywords
- Trichinellosis and other foodborne zoonotic pathogens,
- Trichinella
Rights
Peer review
Yes
Article
- Journal title
- Parasites & Vectors
- Journal volume
- 16
- Article number
- 466 (2023)
- Accepted date
- 2023-10-29
- Submitted date
- 2023-07-06
Citation(s)
Lobanov, V. A., Konecsni, K. A., Scandrett, W. B., & Jenkins, E. J. (2023, December 21). Identification of trichinella taxa by its-1 amplicon next-generation sequencing with an improved resolution for detecting underrepresented genotypes in mixed natural infections - parasites & vectors. BioMed Central. https://doi.org/10.1186/s13071-023-06035-1