Identifcation of Trichinella taxa by ITS-1 amplicon next-generation sequencing with an improved resolution for detecting underrepresented genotypes in mixed natural infections

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DOI

https://doi.org/10.1186/s13071-023-06035-1

Language of the publication
English
Date
2023-12-21
Type
Article
Author(s)
  • Lobanov, Vladislav A.
  • Konecsni, Kelly A.
  • Scandrett, Brad W.
  • Jenkins, Emily J.
Publisher
BioMed Central

Abstract

Amplicon-based next-generation sequencing (NGS) has rapidly gained popularity as a powerful method for delineating taxa in complex communities, including helminths. Here, we applied this approach to identify species and genotypes of zoonotic nematodes of the Trichinella genus. A known limitation of the current multiplex PCR (mPCR) assay recommended by the International Commission on Trichinellosis is that it does not differentiate Trichinella nativa from T. chanchalensis.

Subject

  • Animal diseases

Keywords

  • Trichinellosis and other foodborne zoonotic pathogens,
  • Trichinella

Rights

Peer review

Yes

Article

Journal title
Parasites & Vectors
Journal volume
16
Article number
466 (2023)
Accepted date
2023-10-29
Submitted date
2023-07-06

Citation(s)

Lobanov, V. A., Konecsni, K. A., Scandrett, W. B., & Jenkins, E. J. (2023, December 21). Identification of trichinella taxa by its-1 amplicon next-generation sequencing with an improved resolution for detecting underrepresented genotypes in mixed natural infections - parasites & vectors. BioMed Central. https://doi.org/10.1186/s13071-023-06035-1

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Animals

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