The evolving nature of Bordetella pertussis in Ontario, Canada, 2009-2017 : strains with shifting genotypes and pertactin deficiency
- Download(s)
- DOI
- Language of the publication
- English
- Date
- 2019-07-11
- Type
- Article
- Author(s)
- Raymond S. W. Tsang
- Michelle Shuel
- Kirby Cronin
- Saul Deng
- Kathleen Whyte
- Alex Marchand-Austin
- Jennifer Ma
- Shelly Bolotin
- Natasha Crowcroft
- Kevin Schwartz
- Gary Van Domselaar
- Morag Graham
- Frances B. Jamieson
- Publisher
- Canadian Science Publishing
Abstract
This study examined the evolving nature of Bordetella pertussis in Ontario, Canada, by characterizing isolates for their genotypes and expression of pertactin (PRN). From 2009 to 2017, 413 B. pertussis were cultured from pertussis cases at the Public Health Ontario Laboratory. Their genotypes were determined by partial gene sequence analysis of their virulence and (or) vaccine antigens: filamentous haemagglutinin, PRN, fimbriae 3, and pertussis toxin, including the promoter region. Expression of PRN was measured by Western immunoblot. Two predominant genotypes, ST-1 and ST-2, were found throughout the study and were responsible for 47.5% and 46.3% of all case isolates, respectively. The prevalence of ST-1 appeared to fluctuate from 80.3% in 2009 to 20.0% in 2014 and 58.5% in 2017, while the prevalence of ST-2 changed from 18.4% in 2009 to 80.0% in 2014 and 26.2% in 2017. A PRN-deficient strain was first noted in 2011 (16.7%), and its prevalence increased to 70.8% in 2016 but decreased to 46.2% in 2017. More ST-2 (46.6%) than ST-1 (16.8%) strains were associated with PRN deficiency. Newer ST-21 and ST-22 found in 2015–2017 were uniformly PRN deficient. The impact of the evolving nature of B. pertussis on disease epidemiology requires further longitudinal studies.
Plain language summary
This study examined the evolving nature of Bordetella pertussis in Ontario, Canada, by characterizing isolates for their genotypes and expression of pertactin (PRN). From 2009 to 2017, 413 B. pertussis were cultured from pertussis cases at the Public Health Ontario Laboratory. Two predominant genotypes, Sequence Type (ST)-1 and ST-2, were found throughout the study and were responsible for 47.5% and 46.3% of all case isolates, respectively. The prevalence of ST-1 appeared to fluctuate from 80.3% in 2009 to 20.0% in 2014 and 58.5% in 2017, while the prevalence of ST-2 changed from 18.4% in 2009 to 80.0% in 2014 and 26.2% in 2017. A PRN-deficient strain was first noted in 2011 (16.7%), and its prevalence increased to 70.8% in 2016 but decreased to 46.2% in 2017. More ST-2 (46.6%) than ST-1 (16.8%) strains were associated with PRN deficiency. Newer ST-21 and ST-22 found in 2015–2017 were uniformly PRN deficient. The impact of the evolving nature of B. pertussis on disease epidemiology requires further longitudinal studies.
Subject
- Health,
- Epidemiology,
- Public health,
- Infectious diseases
Keywords
- Bordetella pertussis,
- pertactin deficiency,
- genotype
Rights
Pagination
823-830
Peer review
Yes
Open access level
Gold
Identifiers
- PubMed ID
- 31295416
- ISSN
- 1480-3275
Article
- Journal title
- Canadian Journal of Microbiology
- Journal volume
- 65
- Journal issue
- 11
- Accepted date
- 2019-05-24
Citation(s)
Tsang, R. S. W., Shuel, M., Cronin, K., Deng, S., Whyte, K., Marchand-Austin, A., Ma, J., Bolotin, S., Crowcroft, N., Schwartz, K., Van Domselaar, G., Graham, M., & Jamieson, F. B. (2019). The evolving nature of Bordetella pertussis in Ontario, Canada, 2009-2017: strains with shifting genotypes and pertactin deficiency. Canadian Journal of Microbiology, 65(11), 823–830. https://doi.org/10.1139/cjm-2019-0128