The evolving nature of Bordetella pertussis in Ontario, Canada, 2009-2017 : strains with shifting genotypes and pertactin deficiency

Raymond S. W. Tsang; Michelle Shuel; Kirby Cronin; Saul Deng; Kathleen Whyte; Alex Marchand-Austin; Jennifer Ma; Shelly Bolotin; Natasha Crowcroft; Kevin Schwartz; Gary Van Domselaar; Morag Graham; Frances B. Jamieson

The evolving nature of Bordetella pertussis in Ontario, Canada, 2009-2017 : strains with shifting genotypes and pertactin deficiency

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creativework.keywords - en: Bordetella pertussis; pertactin deficiency; genotype
creativework.keywords - fr: Bordetella pertussis; déficience en pertactine; génotype
dc.contributor.author: Raymond S. W. Tsang; Michelle Shuel; Kirby Cronin; Saul Deng; Kathleen Whyte; Alex Marchand-Austin; Jennifer Ma; Shelly Bolotin; Natasha Crowcroft; Kevin Schwartz; Gary Van Domselaar; Morag Graham; Frances B. Jamieson
dc.date.accepted: 2019-05-24
dc.date.accessioned: 2026-02-17T18:50:40Z
dc.date.available: 2026-02-17T18:50:40Z
dc.date.issued: 2019-07-11
dc.description - en: This study examined the evolving nature of Bordetella pertussis in Ontario, Canada, by characterizing isolates for their genotypes and expression of pertactin (PRN). From 2009 to 2017, 413 B. pertussis were cultured from pertussis cases at the Public Health Ontario Laboratory. Two predominant genotypes, Sequence Type (ST)-1 and ST-2, were found throughout the study and were responsible for 47.5% and 46.3% of all case isolates, respectively. The prevalence of ST-1 appeared to fluctuate from 80.3% in 2009 to 20.0% in 2014 and 58.5% in 2017, while the prevalence of ST-2 changed from 18.4% in 2009 to 80.0% in 2014 and 26.2% in 2017. A PRN-deficient strain was first noted in 2011 (16.7%), and its prevalence increased to 70.8% in 2016 but decreased to 46.2% in 2017. More ST-2 (46.6%) than ST-1 (16.8%) strains were associated with PRN deficiency. Newer ST-21 and ST-22 found in 2015–2017 were uniformly PRN deficient. The impact of the evolving nature of B. pertussis on disease epidemiology requires further longitudinal studies.
dc.description.abstract - en: This study examined the evolving nature of Bordetella pertussis in Ontario, Canada, by characterizing isolates for their genotypes and expression of pertactin (PRN). From 2009 to 2017, 413 B. pertussis were cultured from pertussis cases at the Public Health Ontario Laboratory. Their genotypes were determined by partial gene sequence analysis of their virulence and (or) vaccine antigens: filamentous haemagglutinin, PRN, fimbriae 3, and pertussis toxin, including the promoter region. Expression of PRN was measured by Western immunoblot. Two predominant genotypes, ST-1 and ST-2, were found throughout the study and were responsible for 47.5% and 46.3% of all case isolates, respectively. The prevalence of ST-1 appeared to fluctuate from 80.3% in 2009 to 20.0% in 2014 and 58.5% in 2017, while the prevalence of ST-2 changed from 18.4% in 2009 to 80.0% in 2014 and 26.2% in 2017. A PRN-deficient strain was first noted in 2011 (16.7%), and its prevalence increased to 70.8% in 2016 but decreased to 46.2% in 2017. More ST-2 (46.6%) than ST-1 (16.8%) strains were associated with PRN deficiency. Newer ST-21 and ST-22 found in 2015–2017 were uniformly PRN deficient. The impact of the evolving nature of B. pertussis on disease epidemiology requires further longitudinal studies.
dc.description.abstract-fosrctranslation - fr: Cette étude a examiné la nature évolutive de Bordetella pertussis en Ontario, Canada, en caractérisant les isolats en ce qui concerne leurs génotypes et l’expression de la pertactine (PRN). 413 échantillons de B. pertussis ont été cultivés au Public Health Ontario Laboratory entre 2009 et 2017 à partir de cas de coqueluche. Leurs génotypes ont été déterminés par l’analyse partielle de séquences géniques d’antigènes liés à la virulence ou vaccinaux : l’hémagglutinine filamenteuse, la pertactine, les fimbriae 3 et la toxine pertussique, y compris la région de son promoteur. L’expression de la PRN a été mesurée par immunobuvardage Western. Deux génotypes prédominants, ST-1 et ST-2, ont été trouvés tout au long de l’étude et ceux-ci étaient responsables de 47,5 % et 46,3 % de tous les cas échantillonnés, respectivement. La prévalence de ST-1 semblait fluctuer de 80,3 % en 2009 à 20,0 % en 2014 et 58,5 % en 2017, alors que la prévalence de ST-2 variait de 18,4 % en 2009 à 80,0 % en 2014 et 26,2 % en 2017. Une souche déficiente en PRN a été d’abord observée en 2011 (16,7 % en 2011) et sa prévalence s’est accrue à 70,8 % en 2016 pour ensuite diminuer à 46,2 % en 2017. Davantage de souches ST-2 (46,6 %) que ST-1 (16,8 %) étaient associées à la déficience en PRN. Des souches plus récentes, ST-21 et ST-22 trouvées de 2015 à 2017 étaient systématiquement déficientes en PRN. L’impact de la nature évolutive de B. pertussis sur l’épidémiologie de la maladie demande que de plus amples études longitudinales soient réalisées. [Traduit par la Rédaction]
dc.identifier.citation: Tsang, R. S. W., Shuel, M., Cronin, K., Deng, S., Whyte, K., Marchand-Austin, A., Ma, J., Bolotin, S., Crowcroft, N., Schwartz, K., Van Domselaar, G., Graham, M., & Jamieson, F. B. (2019). The evolving nature of Bordetella pertussis in Ontario, Canada, 2009-2017: strains with shifting genotypes and pertactin deficiency. Canadian Journal of Microbiology, 65(11), 823–830. https://doi.org/10.1139/cjm-2019-0128
dc.identifier.doi: https://doi.org/10.1139/cjm-2019-0128
dc.identifier.issn: 1480-3275
dc.identifier.pubmedID: 31295416
dc.identifier.uri: https://cdnsciencepub.com/doi/10.1139/cjm-2019-0128; https://open-science.canada.ca/handle/123456789/4248
dc.language.iso: en
dc.publisher - en: Canadian Science Publishing
dc.rights - en: Other
dc.rights - fr: Autre
dc.rights.openaccesslevel - en: Gold
dc.rights.openaccesslevel - fr: Or
dc.rights.uri - en: #description-element
dc.rights.uri - fr: #description-element
dc.subject - en: Health; Epidemiology; Public health; Infectious diseases
dc.subject - fr: Santé; Épidémiologie; Public health; Maladie infectieuse
dc.subject.en - en: Health; Epidemiology; Public health; Infectious diseases
dc.subject.fr - fr: Santé; Épidémiologie; Public health; Maladie infectieuse
dc.title - en: The evolving nature of Bordetella pertussis in Ontario, Canada, 2009-2017 : strains with shifting genotypes and pertactin deficiency
dc.type - en: Article
dc.type - fr: Article
local.article.journalissue: 11
local.article.journaltitle - en: Canadian Journal of Microbiology
local.article.journalvolume: 65
local.pagination: 823-830
local.peerreview - en: Yes
local.peerreview - fr: Oui
local.requestdoi - en: No
local.requestdoi - fr: No

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