Fermented blueberry juice extract and its specific fractions have an anti-adipogenic effect in 3 T3-L1 cells

Thumbnail image

Download files

DOI

https://doi.org/10.1186/s12906-016-1519-9

Language of the publication
English
Date
2017-01-06
Type
Article
Author(s)
  • Sánchez-Villavicencio, Mayra L.
  • Vinqvist-Tymchuk, Melinda
  • Kalt, Wilhelmina
  • Matar, Chantal
  • Alarcón Aguilar, Francisco J.
  • Escobar Villanueva, Maria del Carmen
  • Haddad, Pierre S.
Publisher
BioMed Central Ltd.

Abstract

Background: Obesity and Type 2 diabetes have reached epidemic status worldwide. Wild lowbush blueberry (Vaccinium angustifolium Aiton) is a plant of the North American Aboriginal traditional pharmacopeia with antidiabetic potential, especially when it is fermented with Serratia vaccinii. Methods: A phytochemical fractionation scheme was used to identify potential bioactive compounds as confirmed by HPLC retention times and UV-Vis spectra. 3 T3-L1 cells were differentiated for 7 days with either Normal Blueberry Extract (NBE), Fermented Blueberry Extract (FBE/F1), seven fractions and four pure compounds. Triglyceride content was measured. Examination of selected intracellular signalling components (p-Akt, p-AMPK) and transcriptional factors (SREBP-1c and PPARγ) was carried out by Western blot analysis. Results: The inhibitory effect of FBE/F1 on adipocyte triglyceride accumulation was attributed to total phenolic (F2) and chlorogenic acid enriched (F3-2) fractions that both inhibited by 75%. Pure compounds catechol (CAT) and chlorogenic acid (CA) also inhibited adipogenesis by 70%. Treatment with NBE, F1, F3-2, CAT and CA decreased p-AKT, whereas p-AMPK tended to increase with F1. The expression of SREBP1-c was not significantly modulated. In contrast, PPARγ decreased in all experimental groups that inhibited adipogenesis. Conclusions: These results demonstrate that fermented blueberry extract contains compounds with anti-adipogenic activity, which can serve to standardize nutraceutical preparations from fermented blueberry juice and to develop novel compounds with anti-obesity properties.

Subject

  • Health and safety

Keywords

  • Blueberries,
  • Fermented beverages,
  • Beverages--Health aspects,
  • Fat cells

Rights

Pagination

1-9

Peer review

Yes

Open access level

Gold

Identifiers

ISSN
1472-6882

Article

Journal title
BMC Complementary and Alternative Medicine
Journal volume
17
Journal issue
1
Article number
24
Accepted date
2016-12-14
Submitted date
2016-08-24

Citation(s)

Sánchez-Villavicencio, M. L., Vinqvist-Tymchuk, M., Kalt, W., Matar, C., Alarcón Aguilar, F. J., Escobar Villanueva, M. C., & Haddad, P. S. (2017). Fermented blueberry juice extract and its specific fractions have an anti-adipogenic effect in 3 T3-L1 cells. BMC Complementary and Alternative Medicine, 17(1), Article 24. https://doi.org/10.1186/s12906-016-1519-9

Download(s)

URI

Collection(s)

Food and beverages

Full item page

Full item page

Page details

Date modified: