Fermented blueberry juice extract and its specific fractions have an anti-adipogenic effect in 3 T3-L1 cells
- DOI
- Language of the publication
- English
- Date
- 2017-01-06
- Type
- Article
- Author(s)
- Sánchez-Villavicencio, Mayra L.
- Vinqvist-Tymchuk, Melinda
- Kalt, Wilhelmina
- Matar, Chantal
- Alarcón Aguilar, Francisco J.
- Escobar Villanueva, Maria del Carmen
- Haddad, Pierre S.
- Publisher
- BioMed Central Ltd.
Abstract
Background: Obesity and Type 2 diabetes have reached epidemic status worldwide. Wild lowbush blueberry (Vaccinium angustifolium Aiton) is a plant of the North American Aboriginal traditional pharmacopeia with antidiabetic potential, especially when it is fermented with Serratia vaccinii. Methods: A phytochemical fractionation scheme was used to identify potential bioactive compounds as confirmed by HPLC retention times and UV-Vis spectra. 3 T3-L1 cells were differentiated for 7 days with either Normal Blueberry Extract (NBE), Fermented Blueberry Extract (FBE/F1), seven fractions and four pure compounds. Triglyceride content was measured. Examination of selected intracellular signalling components (p-Akt, p-AMPK) and transcriptional factors (SREBP-1c and PPARγ) was carried out by Western blot analysis. Results: The inhibitory effect of FBE/F1 on adipocyte triglyceride accumulation was attributed to total phenolic (F2) and chlorogenic acid enriched (F3-2) fractions that both inhibited by 75%. Pure compounds catechol (CAT) and chlorogenic acid (CA) also inhibited adipogenesis by 70%. Treatment with NBE, F1, F3-2, CAT and CA decreased p-AKT, whereas p-AMPK tended to increase with F1. The expression of SREBP1-c was not significantly modulated. In contrast, PPARγ decreased in all experimental groups that inhibited adipogenesis. Conclusions: These results demonstrate that fermented blueberry extract contains compounds with anti-adipogenic activity, which can serve to standardize nutraceutical preparations from fermented blueberry juice and to develop novel compounds with anti-obesity properties.
Subject
- Health and safety
Keywords
- Blueberries,
- Fermented beverages,
- Beverages--Health aspects,
- Fat cells
Rights
Pagination
1-9
Peer review
Yes
Open access level
Gold
Identifiers
- ISSN
- 1472-6882
Article
- Journal title
- BMC Complementary and Alternative Medicine
- Journal volume
- 17
- Journal issue
- 1
- Article number
- 24
- Accepted date
- 2016-12-14
- Submitted date
- 2016-08-24
Citation(s)
Sánchez-Villavicencio, M. L., Vinqvist-Tymchuk, M., Kalt, W., Matar, C., Alarcón Aguilar, F. J., Escobar Villanueva, M. C., & Haddad, P. S. (2017). Fermented blueberry juice extract and its specific fractions have an anti-adipogenic effect in 3 T3-L1 cells. BMC Complementary and Alternative Medicine, 17(1), Article 24. https://doi.org/10.1186/s12906-016-1519-9