Fermented blueberry juice extract and its specific fractions have an anti-adipogenic effect in 3 T3-L1 cells
Fermented blueberry juice extract and its specific fractions have an anti-adipogenic effect in 3 T3-L1 cells
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- creativework.keywords - en
- Blueberries
- Fermented beverages
- Beverages--Health aspects
- Fat cells
- creativework.keywords - fr
- Bleuets
- Boissons fermentées
- Boissons--Aspect sanitaire
- Adipocytes
- dc.contributor.author
- Sánchez-Villavicencio, Mayra L.
- Vinqvist-Tymchuk, Melinda
- Kalt, Wilhelmina
- Matar, Chantal
- Alarcón Aguilar, Francisco J.
- Escobar Villanueva, Maria del Carmen
- Haddad, Pierre S.
- dc.date.accepted
- 2016-12-14
- dc.date.accessioned
- 2024-10-11T19:07:45Z
- dc.date.available
- 2024-10-11T19:07:45Z
- dc.date.issued
- 2017-01-06
- dc.date.submitted
- 2016-08-24
- dc.description.abstract - en
- Background: Obesity and Type 2 diabetes have reached epidemic status worldwide. Wild lowbush blueberry (Vaccinium angustifolium Aiton) is a plant of the North American Aboriginal traditional pharmacopeia with antidiabetic potential, especially when it is fermented with Serratia vaccinii. Methods: A phytochemical fractionation scheme was used to identify potential bioactive compounds as confirmed by HPLC retention times and UV-Vis spectra. 3 T3-L1 cells were differentiated for 7 days with either Normal Blueberry Extract (NBE), Fermented Blueberry Extract (FBE/F1), seven fractions and four pure compounds. Triglyceride content was measured. Examination of selected intracellular signalling components (p-Akt, p-AMPK) and transcriptional factors (SREBP-1c and PPARγ) was carried out by Western blot analysis. Results: The inhibitory effect of FBE/F1 on adipocyte triglyceride accumulation was attributed to total phenolic (F2) and chlorogenic acid enriched (F3-2) fractions that both inhibited by 75%. Pure compounds catechol (CAT) and chlorogenic acid (CA) also inhibited adipogenesis by 70%. Treatment with NBE, F1, F3-2, CAT and CA decreased p-AKT, whereas p-AMPK tended to increase with F1. The expression of SREBP1-c was not significantly modulated. In contrast, PPARγ decreased in all experimental groups that inhibited adipogenesis. Conclusions: These results demonstrate that fermented blueberry extract contains compounds with anti-adipogenic activity, which can serve to standardize nutraceutical preparations from fermented blueberry juice and to develop novel compounds with anti-obesity properties.
- dc.identifier.citation
- Sánchez-Villavicencio, M. L., Vinqvist-Tymchuk, M., Kalt, W., Matar, C., Alarcón Aguilar, F. J., Escobar Villanueva, M. C., & Haddad, P. S. (2017). Fermented blueberry juice extract and its specific fractions have an anti-adipogenic effect in 3 T3-L1 cells. BMC Complementary and Alternative Medicine, 17(1), Article 24. https://doi.org/10.1186/s12906-016-1519-9
- dc.identifier.doi
- https://doi.org/10.1186/s12906-016-1519-9
- dc.identifier.issn
- 1472-6882
- dc.identifier.uri
- https://open-science.canada.ca/handle/123456789/3058
- dc.language.iso
- en
- dc.publisher
- BioMed Central Ltd.
- dc.rights - en
- Creative Commons Attribution 4.0 International (CC BY 4.0)
- dc.rights - fr
- Creative Commons Attribution 4.0 International (CC BY 4.0)
- dc.rights.openaccesslevel - en
- Gold
- dc.rights.openaccesslevel - fr
- Or
- dc.rights.uri - en
- https://creativecommons.org/licenses/by/4.0/
- dc.rights.uri - fr
- https://creativecommons.org/licenses/by/4.0/deed.fr
- dc.subject - en
- Health and safety
- dc.subject - fr
- Santé et sécurité
- dc.subject.en - en
- Health and safety
- dc.subject.fr - fr
- Santé et sécurité
- dc.title - en
- Fermented blueberry juice extract and its specific fractions have an anti-adipogenic effect in 3 T3-L1 cells
- dc.type - en
- Article
- dc.type - fr
- Article
- local.acceptedmanuscript.articlenum
- 24
- local.article.journalissue
- 1
- local.article.journaltitle
- BMC Complementary and Alternative Medicine
- local.article.journalvolume
- 17
- local.pagination
- 1-9
- local.peerreview - en
- Yes
- local.peerreview - fr
- Oui
- local.requestdoi
- No
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