Host-preferential Fusarium graminearum gene expression during infection of wheat, barley, and maize

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DOI

https://doi.org/10.1016/j.funbio.2015.10.010

Language of the publication
English
Date
2015-11-11
Type
Article
Author(s)
  • Harris, Linda J.
  • Balcerzak, Margaret
  • Johnston, Anne
  • Schneiderman, Danielle
  • Ouellet, Thérèse
Publisher
Elsevier B.V.

Abstract

Fusarium graminearum is a broad host pathogen threatening cereal crops in temperate regions around the world. To better understand how F. graminearum adapts to different hosts, we have performed a comparison of the transcriptome of a single strain of F. graminearum during early infection (up to 4 d post-inoculation) of barley, maize, and wheat using custom oligomer microarrays. Our results showed high similarity between F. graminearum transcriptomes in infected wheat and barley spike tissues. Quantitative RT-PCR was used to validate the gene expression profiles of 24 genes. Host-specific expression of genes was observed in each of the three hosts. This included expression of distinct sets of genes associated with transport and secondary metabolism in each of the three crops, as well as host-specific patterns for particular gene categories such as sugar transporters, integral membrane protein PTH11-like proteins, and chitinases. This study identified 69 F. graminearum genes as preferentially expressed in developing maize kernels relative to wheat and barley spikes. These host-specific differences showcase the genomic flexibility of F. graminearum to adapt to a range of hosts.

Subject

  • Agriculture

Rights

Pagination

111-123

Peer review

Yes

Open access level

Gold

Identifiers

ISSN
1878-6146

Article

Journal title
Fungal Biology
Journal volume
120
Journal issue
1
Accepted date
2015-10-19
Submitted date
2015-07-29

Citation(s)

Harris, L. J., Balcerzak, M., Johnston, A., Schneiderman, D., & Ouellet, T. (2016). Host-preferential fusarium graminearum gene expression during infection of wheat, barley, and maize. Fungal Biology, 120(1), 111–123. https://doi.org/10.1016/j.funbio.2015.10.010

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Collection(s)

Biology

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