Host-preferential Fusarium graminearum gene expression during infection of wheat, barley, and maize

Harris, Linda J.; Balcerzak, Margaret; Johnston, Anne; Schneiderman, Danielle; Ouellet, Thérèse

Host-preferential Fusarium graminearum gene expression during infection of wheat, barley, and maize

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dc.contributor.author: Harris, Linda J.; Balcerzak, Margaret; Johnston, Anne; Schneiderman, Danielle; Ouellet, Thérèse
dc.date.accepted: 2015-10-19
dc.date.accessioned: 2024-07-03T21:40:12Z
dc.date.available: 2024-07-03T21:40:12Z
dc.date.issued: 2015-11-11
dc.date.submitted: 2015-07-29
dc.description.abstract - en: Fusarium graminearum is a broad host pathogen threatening cereal crops in temperate regions around the world. To better understand how F. graminearum adapts to different hosts, we have performed a comparison of the transcriptome of a single strain of F. graminearum during early infection (up to 4 d post-inoculation) of barley, maize, and wheat using custom oligomer microarrays. Our results showed high similarity between F. graminearum transcriptomes in infected wheat and barley spike tissues. Quantitative RT-PCR was used to validate the gene expression profiles of 24 genes. Host-specific expression of genes was observed in each of the three hosts. This included expression of distinct sets of genes associated with transport and secondary metabolism in each of the three crops, as well as host-specific patterns for particular gene categories such as sugar transporters, integral membrane protein PTH11-like proteins, and chitinases. This study identified 69 F. graminearum genes as preferentially expressed in developing maize kernels relative to wheat and barley spikes. These host-specific differences showcase the genomic flexibility of F. graminearum to adapt to a range of hosts.
dc.identifier.citation: Harris, L. J., Balcerzak, M., Johnston, A., Schneiderman, D., & Ouellet, T. (2016). Host-preferential fusarium graminearum gene expression during infection of wheat, barley, and maize. Fungal Biology, 120(1), 111–123. https://doi.org/10.1016/j.funbio.2015.10.010
dc.identifier.doi: https://doi.org/10.1016/j.funbio.2015.10.010
dc.identifier.issn: 1878-6146
dc.identifier.uri: https://open-science.canada.ca/handle/123456789/2650
dc.language.iso: en
dc.publisher: Elsevier B.V.
dc.rights - en: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
dc.rights - fr: Creative Commons Attribution - Pas d'utilisation commerciale - Pas de modification 4.0 International (CC BY-NC-ND 4.0)
dc.rights.openaccesslevel - en: Gold
dc.rights.openaccesslevel - fr: Or
dc.rights.uri - en: https://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.uri - fr: https://creativecommons.org/licenses/by-nc-nd/4.0/deed.fr
dc.subject - en: Agriculture
dc.subject - fr: Agriculture
dc.subject.en - en: Agriculture
dc.subject.fr - fr: Agriculture
dc.title - en: Host-preferential Fusarium graminearum gene expression during infection of wheat, barley, and maize
dc.type - en: Article
dc.type - fr: Article
local.article.journalissue: 1
local.article.journaltitle: Fungal Biology
local.article.journalvolume: 120
local.pagination: 111-123
local.peerreview - en: Yes
local.peerreview - fr: Oui